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The R2-M “Flow through”
hybridization device is a state-
of-the-art liquid handling and
temperature regulating device
for membrane-based affinity
capturing chemical processes
such as DNA hybridization and
protein immunoblot. The “Flow-
through” hybridization
technology overcomes the
limitations caused by diffusion
of reagents in other
membrane-based systems. Using the highly efficient “Flow-
through” hybridization platform
reduces the time for DNA
hybridization reactions from
hours to minutes.
The “Flow-through”
hybridization device eliminates
the need for multiple water
baths, shaking and liquid
handling steps required with
standard DNA hybridization and
protein immunoblot
applications. The closed waste
disposal system contains the
waste solutions avoiding
possible contamination of the
bench top. The operation of the R2-M
device is simple and convenient
– just set the temperature and
add the reagent solutions and
let the device do the rest. The
R2-M device can be operated
manually or by user-defined
programs.
Principle of the patented "Flow-
through" Hybridization
Technology
"Flow-through" Hybridization
Technology provides the
platform for rapid analysis of any
analyte in the solution-Porous-
Matrix System. This is
exemplified by the DNA
hybridization: It is characterized
by active approach to facilitate
the annealing reaction
between the sample molecules
(targets) and the capturing
molecules (probes), which
occur on three-dimensional
porous matrixes rather than
planar surfaces.
The annealing of two
complementary DNA single
strands is a very fast kinetic
process when the two strands
collide. However in the two-
phase conventional
hybridization system, the target
molecules must move toward
the probes fixed in the solid
matrix during hybridization, Hence this diffusion through
solid matrix - a slow process - is
the rate-limiting step and
therefore the incubation step
usually takes hours or even
overnight in order to achieve
optimal results.
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R2-M Flow-through System
